![]() ![]() ![]() G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4 Sticky ends from different EcoO109I sites may not be compatible.Įfficient cleavage requires at least two copies of the NgoMIV recognition sequence.Įfficient cleavage requires at least two copies of the NaeI recognition sequence. Sticky ends from different BlpI sites may not be compatible. Sticky ends from different BtgI sites may not be compatible. PaeR7I does not recognize the sequence CTCTCGAG.
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